The Czech Republic national under-21 football team is the national under-21 association football team of the Czech Republic and is controlled by the Football Association of the Czech Republic. The team competes in the UEFA European Under-21 Championship, held every two years.

Although the breakup of Czechoslovakia occurred officially on 1 January 1993, the under-21 team continued until the end of the 1994 championship. After that, the Czech Republic and the Slovakia under-21s became separate footballing entities.

For both nations, the first matches were played in September 1994 in qualification for the 1996 championship.

The Czech Republic under-21 team reached the quarter-finals in 1996, but failed to qualify for 1998. The team reached the final in both the 2000 and 2002 tournaments, winning the latter on penalties.

Subsequently, the team failed to qualify for the tournaments in 2004 and 2006. They qualified for the 2007 competition but finished last in the group stage. They once again failed to qualify for the 2009 competition. In the 2011 UEFA European Under-21 Championship, they placed fourth.

Tšehhi U21 jalgpallimeeskond esindab Tšehhit rahvusvahelistel jalgpallivõistlustel ja sõprusmängudes. Meeskonda haldab Tšehhi Jalgpalliliit.